Analysis of short tandem repeats by using SERS monitoring and electrochemical melting.

Strain discrimination of Yersinia pestis using a SERS-based electrochemically driven melting curve analysis of variable number tandem repeat sequences.

Strain discrimination within genetically highly similar bacteria is critical for epidemiological studies and forensic applications. An electrochemically driven melting curve analysis monitored by SERS has been utilised to reliably discriminate strains of the bacterial pathogen Yersinia pestis, the causative agent of plague. DNA amplicons containing Variable Number Tandem Repeats (VNTRs) were ge...

Editorial: Could Speciation Across Evolution be Governed by Genetic Switch Codes at Short Tandem Repeats?

Segmental Duplications as a Complement Strategy to Short Tandem Repeats in the Prenatal Diagnosis of Down Syndrome

Background: Quantitative fluorescence-polymerase chain reaction (QF-PCR) is an inexpensive and accurate method for the prenatal diagnosis of aneuploidies that applies short tandem repeats (STRs) as a chromosome-specific marker. Despite its apparent advantages, QF-PCR is not applicable in all cases due to the presence of uninformative STRs. This study was carried out to investigate the efficienc...

Strain discrimination of Yersinia pestis using a SERS-based electrochemically driven melting curve analysis of variable number tandem repeat sequences† †Electronic supplementary information (ESI) available: Electrochemical melting curves along with 95% confidence intervals for the three PCR amplicons hybridized to probe-1, fluorescence melting curves for amplicons hybridized to probe-2 in the presence of a blocker oligonucleotide, structure of the thiol linker. See DOI: 10.1039/c4sc03084b

Chemistry, University of Southampton, H E-mail: [email protected] ATDBio Ltd, Chemistry, University of Sout 1BJ, UK DSTL, Wiltshire SP4 0JQ, Salisbury, Porton Department of Chemistry, University of O Oxford OX1 3TA, UK † Electronic supplementary information ( curves along with 95% condence int hybridized to probe-1, uorescence melti probe-2 in the presence of a blocker o linker. See DOI: 10.103...

Molecular Genetic Analysis of the Variable Number of Tandem-Repeat Alleles at the Phenylalanine Hydroxylase Gene in Iranian Azeri Turkish Population

Background: The variable numbers of tandem-repeat (VNTR) alleles at the phenylalanine hydroxylase (PAH) gene have been used in carrier detection and prenatal diagnosis in phenylketonuria families. This study was carried out to analyze VNTR alleles at the PAH gene in Iranian Azeri Turkish population. Methods: In this study, 200 alleles from general population were studied by PCR. Results: The fr...